Python Reference

Environment variables, advanced configuration, and deployment reference for Python apps on Galaxy.

Environment Variables

Set these in the Galaxy deployment form or your app's Settings page.

Variable	Required	Default	Description
`PORT`	Yes	5000	Port your app listens on. Galaxy assigns this automatically.
`PYTHONUNBUFFERED`	Yes	(none)	Set to `1` to ensure immediate output flushing
`PYTHON_VERSION`	No	(none)	Specify Python version if needed

Variable	Default	Description
`DEBUG`	`false`	Enable debug mode (set to `0` for production)
`LOG_LEVEL`	`info`	Logging verbosity
`TZ`	`UTC`	Timezone for your app

Most Python apps don't need a build step. If yours does, set it in Galaxy:

Build Command: Custom command (e.g., python manage.py collectstatic --noinput)
Start Command: gunicorn app:app or python main.py

Galaxy runs install, then build (if set), then start.

Create a simple health check endpoint for Galaxy to monitor:

@app.route('/health')
def health():
    return {
        'status': 'ok',
        'timestamp': datetime.now().isoformat()
    }, 200

In the Galaxy deployment form, set Health Check Path to /health.

Galaxy supports Python 3.10, 3.11, 3.12, 3.13, and Python 2.7 (legacy). For best compatibility, use Python 3.12 or 3.13.

Select your Python version via the Docker Preset option during deployment or in your app's Settings page.

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